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SAI's Alumina for chromatography has contributed to
the continued growing use of this material, in a diverse number of applications. Indeed,
the major reasons for this growth has been our contribution to the standardization of the
manufacturing process resulting in standardized grades of Alumina with very well
controlled and defined:
- Activity (according to Brockmann):
Super Activity I
Standard (Std) Activity I-IV
- pH: Acid(A) Basic(B) Neutral(N)
- Stepless Deactivation behavior
- Constant Deactivation behavior
- Controlled Chromatographic Parameters
- Controlled Surface Area, Porosity
Scientific Adsorbents' Aluminas
Analytical, Prep LC, Sample Processing
Super I
Super Activity I Aluminas are as unique as SAI's
products. They demonstrate approximately twice the capacity as compared to Standard
Activity I. Surface modifications available are "A" (Acid), "B"
(Basic), and "N" (Neutral). Super Activity I Aluminas constitute the starting
material for the Scientific Adsorbent's line of Aluminas. Therefore, it is easy to change
between various modes of chromatography. A special feature of Super Activity I is
absolutely constant deactivation behavior which is valid for the deactivation process as
well as when in contact with the chromatographic solvent.
CAT# |
Description |
4601-05 |
ALUMINA A, SUPER I, 50-200u, 500g |
04601-1 |
ALUMINA A, SUPER I, 50-200u, 1kg |
04601-5 |
ALUMINA A, SUPER I, 50-200u, 5kg |
04601-50 |
ALUMINA A, SUPER I, 50-200u, 50kg |
04577-05 |
ALUMINA B, SUPER I, 50-200u, 500g |
04577-1 |
ALUMINA B, SUPER I, 50-200u, 1kg |
04577-5 |
ALUMINA B, SUPER I, 50-200u, 5kg |
04577-50 |
ALUMINA B, SUPER I, 50-200u, 50kg |
04589-05 |
ALUMINA N, SUPER I, 50-200u, 500g |
04589-1 |
ALUMINA N, SUPER I, 50-200u, 1kg |
04589-5 |
ALUMINA N, SUPER I, 50-200u, 5kg |
04589-50 |
ALUMINA N, SUPER I, 50-200u, 50kg |
Standard Activity I
Alumina Standard Activity I (Act I) is available with various surface modifications, A, B,
and N to facilitate the separation of a wide range of compounds. In addition to pH, the
activity of the surface of Alumina can mediate the separation. It is simple to adjust the
activity by adjusting the water content of the material. (Alternatively, other polar media
can replace the water.)
Use high activity Alumina (Std Act I, Super
Act I) for the separation of polar samples in nonpolar solvent systems... and for the
purification of solvents
Use lower activity Alumina for less polar
samples
CAT# |
Description |
02159-05 |
ALUMINA A, ACT I, 50-200u, 500g |
02159-1 |
ALUMINA A, ACT I, 50-200u, 1kg |
02159-5 |
ALUMINA A, ACT I, 50-200u, 5kg |
02159-50 |
ALUMINA A, ACT I, 50-200u, 50kg |
02078-05 |
ALUMINA B, ACT I, 50-200u, 500g |
02078-1 |
ALUMINA B, ACT I, 50-200u, 1kg |
02078-5 |
ALUMINA B, ACT I, 50-200u, 5kg |
02078-50 |
ALUMINA B, ACT I, 50-200u, 50kg |
02135-05 |
ALUMINA N, ACT I, 50-200u, 500g |
02135-1 |
ALUMINA N, ACT I, 50-200u, 1kg |
02135-5 |
ALUMINA N, ACT I, 50-200u, 5kg |
02135-50 |
ALUMINA N, ACT I, 50-200u, 50 kg |
DCC Alumina
DCC - Dry Column Chromatography is a versatile Prep LC method that bridges the
gap between analytical TLC and preparative column chromatography. See Silica DCC for detailed procedure.
CAT# |
Description |
04514-05 |
ALUMINA DCC, 63-200u, 500g |
04514-5 |
ALUMINA DCC, 63-200u, 5kg |
04514-50 |
ALUMINA DCC, 63-200u, 50kg |
"Flash" Alumina
Flash Chromatography is a rapid Prep LC technique that facilitates the separation of 0.1 -
10 g of material via simple economical laboratory protocols.
CAT# |
Description |
02061-05 |
ALUMINA N, 40 MICRONS(FLASH), 32-63u, 500g |
02061-1 |
ALUMINA N, 40 MICRONS(FLASH), 32-63u, 1kg |
02061-5 |
ALUMINA N, 40 MICRONS(FLASH), 32-63u, 5kg |
02062-05 |
ALUMINA N, ACTIVE, 40 MICRONS(FLASH),
32-63u, 500g |
Activity II, III
Alumina II - III sorbents are economical adsorbents of medium activity. Use this material
for general purpose scouting and in cases where the use of carbon black is precluded due
to its organic nature. Also, use Alumina II - III as a replacement for organic/polymeric
ion exchangers, especially when it is necessary to overcome temperature and radiation
cleavage problems.
CAT# |
Description |
04694-05 |
ALUMINA ACT II-III, 50-200u, 500g |
04694-5 |
ALUMINA ACT II-III, 50-200u, 5kg |
04694-50 |
ALUMINA ACT II-III, 50-200u, 50kg |
Alumina for HPLC
The following Catalog #s are recommended:
CAT# |
Description |
02142 |
ALUMINA N, 5u, 10g |
02143 |
ALUMINA N, 5u, 100g |
02148 |
ALUMINA N, 10u, 10g |
02149 |
ALUMINA N, 10u, 100g |
02151 |
ALUMINA N, 15u, 10g |
02152 |
ALUMINA N, 15u, 100g |
02156 |
ALUMINA N, 25u, 10g |
02157 |
ALUMINA N, 25u, 100g |
02063 |
ALUMINA A, ACTIVE, 18-32u, 100g |
02065 |
ALUMINA B, ACTIVE, 18-32u, 100g |
02058 |
ALUMINA N, ACTIVE, 18-32u, 10g |
02059 |
ALUMINA N, ACTIVE, 18-32u, 100g |
Alumina Oxides for Thin-Layer Chromatography
The following Catalog #s are recommended:
CAT# |
Description |
04347-1 |
ALUMINA A-TLC, 1kg |
04347-50 |
ALUMINA A-TLC, 50kg |
04341-1 |
ALUMINA B-TLC, 1kg |
04341-50 |
ALUMINA B-TLC, 50kg |
04344-1 |
ALUMINA N-TLC, 1kg |
04344-50 |
ALUMINA N-TLC, 50kg |
04413-1 |
ALUMINA G-TLC, 1kg |
04413-50 |
ALUMINA G-TLC, 50kg |
Deactivation
Protocols
Introduction
SAI's Aluminas are unique products, i.e. Super I, Std Act I, etc. High activity Alumina
can be used for polar samples in nonpolar solvents, and for the purification of solvents.
Lower activities of Alumina can readily be obtained by the addition of polar media,
especially water. Thus each problem can be discretely resolved via the adjustment of each
sorption system.
Special Features
Super I Aluminas show an approximate double capacity as compared to Activity I. Super I
does not have to be deactivated in steps. By following the appropriate deactivation
curves, deactivation can be achieved in minute increments.
Deactivation behavior
By following the procedures below, it is relatively easy to obtain the desired activity.
Deactivation
Behavior - Alumina |
Activity Grade |
Alumina Type |
Super I |
I |
II |
III |
IV |
V |
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Super I - A,B,N |
0 |
1 |
4 |
7 |
10 |
19 |
% H2O Added |
Std Act I - A,B,N |
NA |
0 |
3 |
6 |
10 |
15 |
% H2O Added |
A = Acid, B
= Base, N = Neutral |
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Deactivation Procedure(s)
The % water addition shown above is based upon weight / weight relationships; these
relationships are critical and any deviation will/could result in obtaining improper
activities.
To reproduce the desired activity, weigh an appropriate amount of
Alumina into a glass bottle. Add the appropriate weight of water to the Alumina and close
the bottle with a stopper. For example, 97 g of Alumina + 3 g H2O = 3% water
addition.
Shake well until all lumps disappear. Wait until the mixture has
cooled to room temperature. Keep the container closed so that equilibrium conditions
remain constant.
Application:
Typical Chromatography Uses of Alumina |
Application |
Recommended Alumina |
ALKALOIDS
Isolation from ergot-, opium-, rauwolfia-, and other alkaloids |
Basic, medium activity, Specialty |
ANTIBIOTICS
Isolation, purification |
Neutral |
ESSENTIAL OILS
Removal of terpenes |
Basic, Neutral |
PLANT EXTRACTION
Isolation of active substances |
Basic, Neutral, Acid |
DEHYDRATION OF ORGANIC SOLVENTS |
Basic, highly active |
ENZYMES
Purification |
Neutral |
GLYCOSIDES
Isolation of digitalis-, strophantus-, glycosides, etc. |
Neutral |
REMOVAL OF PEROXIDES
from organic solvents |
Basic, highly active |
HORMONES
Isolation and purification of synthetic products, of ketosteroids from neutral materials,
etc. |
Neutral |
PURIFICATION OF ORGANIC SOLVENTS
for analytical and technical purposes |
Basic, highly active |
OILS
Clarification of fatty oils, separation of fatty acids |
Basic |
PCB's
Removal from solvents, transformer oils |
Alumina "C"
CAT#:
02103 |
ALUMINA C (PCB), 50kg |
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REMOVAL OF PYROGENS
from injectable solutions and infusions |
Alumina "P"
CAT#:
02120 |
ALUMINA P (PYROGEN), 50kg |
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